Part:BBa_K3796002:Design

capA from Bacillus subtilis str.168

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 364
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 364
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 364
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 364
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

A segment of the RBS recognized by the ribosome of Corynebacterium glutamicum and a promoter recognized by the sigma factor needs to be attached upstream of this sequence, as well as a segment of the terminator downstream.

Source

Bacillus subtilis str.168 genome